How to Troubleshoot Genotype
Instructions
-
-
1
Ensure the equipment you are using is up to laboratory standards in order to avoid incorrect or corrupted readings. This means washing and sterilizing pipettes as well as cleaning your workspace and using proper laboratory gloves as you carry out this work.
-
2
If you are having trouble with the PCR product, run a different PCR reaction for each primer to solve the problem.
-
3
Troubleshoot the problem of bands failing to amplify by changing the temperature of the experiment.
-
4
Buy or order new primers. It can often be the case that your simply have a primer that is not fit for function, in which case it must be replaced.
-
5
Purify the DNA specimen if your other methods of troubleshooting fail to reap rewards. This can be done through sodium hydroxide extraction.
-
1