What is a Positive test for reducing sugar results?
Benedict's Test- Benedict's qualitative reagent is a deep blue solution.
- Positive result: When reducing sugars oxidizes and reduces the cupric ions in Benedict's reagent to cuprous ions, the color changes from blue to green to yellow to orange, or even red-orange, indicating the presence of reducing sugar.
Fehling's Test
- Fehling's qualitative reagent consists of two solutions, Fehling's A and Fehling's B, which are mixed in equal volumes before performing the test.
- Positive result: When reducing sugar reduces cupric ions in Fehling's reagent to cuprous oxide, a red-orange precipitate forms, indicating the presence of reducing sugars.
Barfoed Test
- Barfoed's reagent is a solution containing copper acetate and acetic acid.
- Positive result: A red precipitate of cuprous oxide is formed, indicating the presence of reducing sugar.
Nylander's Test
- Nylander's reagent consists of bismuth subnitrate, sodium potassium tartrate, and sodium hydroxide in solution.
- Positive result: A black or gray precipitate forms when reducing sugars react with bismuth subnitrate to produce elemental bismuth.
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