What is make plasma hemolysis inlavender tube?

Lavender top tubes contain ethylenediaminetetraacetic acid (EDTA) as an anticoagulant. EDTA binds calcium ions, which are necessary for the clotting cascade, thus preventing blood from clotting.

Plasma hemolysis occurs when red blood cells (RBCs) rupture and release their contents into the plasma. This can cause the plasma to appear pink or red, and can interfere with laboratory tests that measure the concentration of analytes in the plasma.

There are a number of factors that can cause plasma hemolysis, including:

* Excessive agitation or shaking of the blood collection tube. This can damage the RBCs and cause them to rupture.

* Collection of blood from a vein that is too small. This can cause the blood to flow too slowly into the tube, which can also damage the RBCs.

* Inadequate mixing of the blood with the EDTA. This can prevent the EDTA from effectively binding calcium ions and can lead to clotting of the blood.

* Storage of the blood collection tube at too high or too low a temperature. This can also damage the RBCs and cause hemolysis.

To avoid plasma hemolysis, it is important to follow the manufacturer's instructions for the collection and handling of blood in EDTA tubes.