What is non - hemolysis 10?

Non-Hemolysis 10 refers to a specific criterion used in testing for blood compatibility. It is part of the compatibility testing process performed prior to blood transfusions to ensure that the donor's blood is compatible with the recipient's blood.

What does Non-Hemolysis 10 mean?

- Non-Hemolysis: This indicates that there is no visible destruction or breakdown of red blood cells (hemolysis) when the recipient's serum (or plasma) is mixed with the donor's red blood cells.

- 10: The number 10 refers to the serum dilution used in the compatibility test. A 10% serum dilution is commonly employed in compatibility testing.

Why is Non-Hemolysis Important?

In compatibility testing, it's crucial to determine whether the recipient's serum contains antibodies that can recognize and bind to specific antigens on the donor's red blood cells. If such binding occurs, it could lead to hemolysis, which can cause severe adverse reactions in the recipient.

A negative Non-Hemolysis 10 result suggests that no incompatible antibodies are present in the recipient's serum that would cause hemolysis of the donor's red blood cells. This indicates a lack of clinically significant red cell antibodies that could pose a risk during the transfusion.

Test Procedure

To perform the Non-Hemolysis 10 test, a sample of the recipient's serum is diluted with saline solution (typically 1:10 dilution). The diluted serum is then mixed with the donor's red blood cells and incubated at a specific temperature for a designated time. After incubation, the mixture is examined visually to assess whether hemolysis has occurred.

Clinical Significance

A positive Non-Hemolysis 10 result indicates hemolysis, suggesting that incompatible antibodies are present in the recipient's serum. In such cases, further compatibility testing and antibody identification would be necessary to determine the specific antibodies involved. This would guide the selection of appropriate, compatible blood for transfusion.

By ensuring Non-Hemolysis 10 (and other compatibility criteria), blood transfusions can be performed safely, minimizing the risk of hemolytic transfusion reactions and other adverse outcomes.