Which stain would be the best choice for detecting mycobacterium bacteria responsible tuberculosis and leprosy?
The Ziehl-Neelsen (ZN) stain is the best choice for detecting mycobacterium bacteria responsible for tuberculosis and leprosy. The ZN stain is a type of acid-fast stain, which means that it can be used to stain bacteria that have a high lipid content in their cell walls. Mycobacterium bacteria have a high lipid content in their cell walls, which makes them resistant to the decolorizing agent that is used in the ZN stain. This means that Mycobacterium bacteria will retain the primary stain (carbol fuchsin) and appear red when viewed under a microscope, while other bacteria will be decolorized and appear blue. The ZN stain is a widely used and effective method for detecting Mycobacterium bacteria in clinical specimens.
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