Is a sterile item free from all microorganisms and their spores?

Yes, a sterile item is free from all microorganisms and their spores. Sterilization is the process of eliminating all forms of microbial life, including vegetative bacteria, bacterial spores, fungi, and viruses. It is crucial in healthcare and various industries to ensure the safety and effectiveness of medical devices, surgical instruments, pharmaceuticals, and other products susceptible to microbial contamination.