How to Prepare Glucose Oxidase
Things You'll Need
- Beakers
- Potassium phosphate (KH2PO4)
- Distilled water
- Stir plate
- pH meter
- 1M Potassium hydroxide solution
- Glucose oxidase
- Test tubes (optional)
Instructions
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1
Prepare a 0.1M potassium phosphate buffer solution by adding 13.6 mg Potassium Phosphate (KH2PO4) into 800 ml of distilled water. Use 1M Potassium Hydroxide to adjust the pH to 7.0 then add enough distilled water to bring the volume to 1 L.
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2
Add 10mg of glucose oxidase per 1ml of distilled water in a separate beaker. Mix with gentle shaking and swirling to avoid foaming.
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3
Add the glucose oxidase to the 0.1M potassium phosphate buffer in separate beakers or test tubes in the various concentrations needed for your assay. You will need to calculate the concentrations you need based on the detection levels you're trying to analyze and the number of different concentrations you want for your linear regression calculation.
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