Will prolonged application of tourniquet affect k?

Yes, prolonged application of a tourniquet can affect the k value of hemoglobin. Hemoglobin is the oxygen-carrying protein in red blood cells, and its affinity for oxygen is measured by the k value. Prolonged application of a tourniquet can lead to tissue hypoxia, which can cause the red blood cells to lose their oxygen. This can lead to a decrease in the k value and a decrease in the oxygen-carrying capacity of the blood.