Why do you isolate from sections obtained at the advancing area of lesion?

There are several reasons why it is important to isolate from sections obtained at the advancing area of a lesion when performing microbiological investigations:

Increased likelihood of obtaining the causative agent: The advancing area of a lesion often represents the site of active infection or invasion by microorganisms. By isolating from this area, there is a higher probability of recovering the causative agent responsible for the lesion.

Minimized contamination: Isolating from the advancing area reduces the risk of contamination from surrounding tissues or the environment. By obtaining a sample from a deeper, less-contaminated area, the likelihood of isolating the true pathogen is increased.

Accurate diagnosis and targeted treatment: Isolate obtained from the advancing area can provide more accurate information for diagnostic purposes. By identifying the specific pathogen responsible for the lesion, appropriate and targeted treatment can be initiated, improving the chances of a successful outcome.

Understanding the nature of the infection: Isolating from the advancing area can also provide insights into the nature and progression of the infection. Studying the characteristics and behavior of the pathogen can aid in determining the virulence, transmissibility, and potential reservoirs of the infection, which is essential for effective infection control and prevention strategies.