Is it possible for a pregnancy test to be negative if taken at night but still pregnant?

Pregnancy tests are most accurate when taken first thing in the morning, as the concentration of the pregnancy hormone, human chorionic gonadotropin (hCG), is at its highest at this time. However, it is still possible to get an accurate result if you take a pregnancy test in the evening, as long as you are at least a week late for your period. If you take a pregnancy test too early, the hCG levels in your urine may not be high enough to be detected by the test, even if you are pregnant. In this case, you should repeat the test in a few days or a week to confirm the result.