DNA Testing Sample Requirements

DNA samples are blood samples or other biological specimens provided by individuals for DNA analysis. DNA samples are commonly used in the medical field to diagnose genetic disorders such as inherited diseases. They are also used by the police to investigate criminal cases through the process of matching the samples with evidence collected from a crime scene such as blood or hair. In the United States the collection, analysis, and use of genetic information is regulated by the Genetic Information Non-Discrimination Act.

DNA Mass Requirements

• The total mass of DNA sample required involves the mass for re-analyses and the mass for pipetting, the process of collecting a measured quantity of DNA source such as saliva from which the DNA will be extracted for analysis. It is done using a laboratory instrument called a pipette, designed to collect tiny liquid volumes. Therefore, the appropriate DNA mass depends on the scope of your project activities. For instance, for Illumina Infinium HD genotyping, a mass of 200 nanograms (ng) per sample should be collected. For Illumina Golden Gate genotyping, a mass of 250 ng per sample is required.

DNA Volume Requirements

• For DNA analysis, the minimum volume required for successful analysis is 5 microliters (ul). However, the collection of appropriate sample volumes depends on the concentration and the required mass of sample for analysis. For samples that are highly concentrated, such that the volume required is less than 5 ul, the sample should be diluted until the volume becomes 5 ul. If the DNA sample is being transported for a long distance, the best way is to transport it as a dried sample.

DNA Concentration Range

• DNA samples can be submitted as wet or dry samples. The concentration of DNA samples depends on whether they are wet or dry. For wet DNA samples, the minimum concentrations for Uniplex and Sequenom iPLEX assays is 5ng/ul whereas that for Illumine genotyping is 50.0 ng/ul. DNA samples should be as concentrated as possible. If dilution is necessary, only water should be used to dilute the samples.

Sample Labeling

• The collected sample should be labeled with the patient's names and date of birth. A requisition form should be filled and signed with the consent of the patient, and the form together with the DNA sample should be sent to the laboratory where analysis will be done.

DNA Sample and Plate Requirements

• When submitting samples, only the 96 well-plates should be used. These plates should be sealed off completely to avoid contamination during transportation. The mass, volume, and concentration of all samples should be uniform and sufficient to enable a successful analysis. In cases where either one of these factors is varying, the DNA samples will be disqualified for analysis.