How to: CNS Map to CCP4

CNS is a group of programs created by a group of universities. It analyzes and creates maps for crystallography and NMR in the medical field. Another program that performs this same function is CCP4. However, these two programs save their files using two different extensions. Converting CNS maps to CCP4 maps requires understanding how to use USF's MAPMAN program. You must be able to the use the command line in this program to type the specific, necessary commands.

Instructions

• 1

  Open MAPMAN and select the "Command Line" option from the top menu. This opens up a typing based command line programming method.

• 2

  Type "read m1 ${prefix}_1fofc.map CNS" into the first line of command. Replace the "${prefix}" line with the base path and the file name of your CNS map. Press the "Enter" button to go to the next line.

• 3

  Enter the line "read m2 ${prefix}_2fofc.map CNS" and enter the base path and file name of the second map you want to convert. Press "Enter" to go to the next line. Add as many maps as you want to convert.

• 4

  Type "write m1 ${prefix}_1fofc.map CCP4" on the next line, replacing "${prefix}" with the base path and file name to which you want to save your converted map. Press "Enter" and add a new line in the same way for each map you are converting.

• 5

  Input "quit" as your last line and press "Enter" wait for your program to run and then check the base path to which you saved your converted maps. Make sure your converted maps were saved here. Open these new maps with CCP4.