How to Prepare a Hemoglobin Specimen for Electrophoresis
Things You'll Need
- Hemoglobin sample (from blood)
- Eppendorf tubes
- Laboratory freezer at -20 degrees centrigrade
- Gel Electrophoresis kit
Instructions
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Preparation
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1
Transfer the whole blood sample to be used for electrophoresis into an eppendorf tube. Ensure the tube is properly closed.
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2
Freeze this sample in a freezer suitable for the storage of organic materials such as blood, at -20 degrees centrigrade. Store until completely frozen (a few hours or perhaps overnight).
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3
Defrost the sample, without vortexing or mixing, and remove the supernatant; this is the hemolysate that will be used for electrophoresis.
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4
Add the buffers most suited to the gel and electrophoresis conditions to be to run the supernatant sample.
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5
Load and run the gel electrophoresis experiment according to the equipment and gel being used.
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