How would the IMVic tests differentiate E. coli from Enterobacter aerogenes?
The IMViC tests (indole, methyl red, Voges-Proskauer, and citrate utilization) are a series of biochemical tests used to differentiate between different species of bacteria.
Indole test:
- E. coli: positive (produces indole)
- Enterobacter aerogenes: negative (does not produce indole)
Methyl red test:
- E. coli: negative (does not produce sufficient acid to turn the indicator red)
- Enterobacter aerogenes: positive (produces sufficient acid to turn the indicator red)
Voges-Proskauer test:
- E. coli: negative (does not produce acetoin)
- Enterobacter aerogenes: positive (produces acetoin)
Citrate utilization test:
- E. coli: positive (can utilize citrate as a sole carbon source)
- Enterobacter aerogenes: negative (cannot utilize citrate as a sole carbon source)
In summary, the IMViC tests can be used to differentiate E. coli from Enterobacter aerogenes based on their different reactions in these tests. E. coli is indole-positive, methyl red-negative, Voges-Proskauer-negative, and citrate-positive, while E. aerogenes is indole-negative, methyl red-positive, Voges-Proskauer-positive, and citrate-negative.