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Autoclave Information

Autoclaves are devices able to withstand high pressure and temperatures. They use saturated steam concentrated under high pressure to effectively sterilize materials. Autoclaves usually feature indicators that let the operator know when heat and pressure have reached effective levels.

  1. Applications

    • There are several practical applications for autoclaves. Different types of autoclaves are widely used in chemical industries to create certain dyes as well as facilitate specific chemical reactions that require high pressure. In the medical field, autoclaves are used to sterilize surgical instruments. The instruments are placed in water within an autoclave and the water is heated under pressure above its boiling point, creating an environment that discourages bacterial growth and sterilizes the instruments.

    Function

    • For the proper functioning of an autoclave, internal temperatures must reach at least 250 degrees Fahrenheit. The minimum autoclaving time required for biohazard waste is around 45 minutes after temperatures reach 250 degrees Fahrenheit and 15 pounds per square inch. Materials that have higher concentrations of unwanted organisms will take longer to sterilize. Saturating the load with steam is essential to effectively decontaminate it. It will take an autoclave longer to reach desired temperatures and pressures if the load is densely arranged.

    Safety

    • Safety equipment such as eye protection, heat-resistant gloves, lab coats and closed-toe shoes should be worn while operating an autoclave. Before opening the door of an autoclave, the operator needs to make sure that pressure within the device is near zero, to prevent steam burns. The steam within an autoclave should be let out slowly and materials within the autoclave should be given at least 10 minutes to cool before handling.

    Warnings

    • Sealed containers are not to be placed in autoclaves because they may explode under the heat and pressure. Solvents, corrosive chemicals such as chloroform, and radioactive materials should never be used in autoclaves. To ensure the proper functioning of an autoclave, schedule regular inspections by qualified professionals.

    Steam Cooker

    • A steam cooker is a device used in kitchens that operates under the same principles as an autoclave. It cooks various foods under heat and pressure. The idea of the pressure cooker was adapted by Denis Papin in 1679. The device has become widely used since its creation.

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